This thesis focuses on motivation and motivation theories and their application in the business of a selected segment of the company. Through the thesis, the theoretical part presents these explained theories of motivation and Myers-Briggs testing (personality test) in order to better understand the human personality and its impact on motivation. The paper also presents the results of a questionnaire and an accompanying telephone conversation based on which opinions on motivation and the impact of motivational methods on employees of the Online Department and their attitude towards the Myers-Briggs personality test and the use of it and similar personality tests to motivate employees. The basic goal of the research is to determine the influence of employees' personalities on the distribution of jobs, motivating and guiding employees, and on the efficiency and productivity of their work. The research is focused on the influence of the motivation of the leadership of the observed segment of the company on the quality of work and the relationship between the effectiveness of the method of motivation and the type of personality of employees. The survey questionnaire and research took into account the current epidemiological situation and the consequences of the current pandemic, and the survey was conducted remotely and accompanied by a telephone conversation, and part of it also focused on the impact of COVID-19 virus on job security and employee satisfaction and motivation. The results of the research show that employees are currently satisfied with the way they're being motivated, but believe that personality has an impact on motivation and that in this area in their department, or the observed segment of the company in which they work, there is room for improvement. Employees mostly believe that they are motivated to do their job to the best of their ability and that the job security provided by their current employer and the accompanying working conditions from home, which are an applied factor due to the current epidemiological situation, further affect their satisfaction and motivation to perform daily tasks.