Organizational culture and its impact on employee satisfaction were elaborated in detail and a study was conducted on the impact of organizational culture on employee satisfaction based on ALFA ATEST d.o.o.’s example. The complexity of organizational culture has been studied by scientists, and some of the many definitions of organizational culture, as well as its functions and dimensions, are outlined in the paper. An organization’s culture classification shows different types of the culture and defines the elements that characterize a particular type of culture. The classification of culture is the first step in the process of identifying the type of culture that prevails in an organization, and in its subsequent adoption or change in order to make progress. Employee motivation and satisfaction, as an integral part of organizational culture, are an important task of management. Their impact on employee productivity and organizational performance is significant. Basic human needs and impulses that need to be met to maximize work efficiency are shown through basic motivational processes and Maslow’s hierarchy of needs. Measuring organizational culture at ALFA ATEST d.o.o. was performed using an OCAI instrument and a marketing culture characterized by a result-oriented, market-oriented and demanding leadership of the organization was found to be the dominant type of culture. A desirable form of organizational culture is different from the current one, and that is a clan organizational culture characterized by a friendly, family-like and very relaxed atmosphere where executives have the role of a mentor who supports its employees. A survey was conducted among the members of the selected company using an anonymous questionnaire and, analyzing the factors respondents’ motivation and satisfaction, it was determined that organizational culture has a considerable impact on employee satisfaction at work.